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Iranian Journal of Microbiology، جلد ۶، شماره ۴، صفحات ۲۷۶-۲۸۰
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عنوان انگلیسی Survey on O157:H7 enterohemorrhagic Escherichia coli (EHEC) in cattle in Golestan province, Iran.
چکیده انگلیسی مقاله Background and Objectives: A diverse group of Escherichia coli are known as enterohemorrhagic Escherichia coli (EHEC) including O157:H7 and non-O157 EHEC. Enterohemorrhagic strains are related to severe clinical conditions in humans including hemorrhagic colitis and hemolytic uremic syndrome, and most of the recorded outbreaks occurred due to O157: H7 E. coli . The aim of the present study was to investigate the presence of O157:H7 E. coli among healthy cattle in Golestan province. Materials and Methods: Fecal samples were collected from 180 clinically healthy cattle in Golestan province. After primary enrichment, samples were streaked on sorbitol MacConkey agar supplemented with cefixime and potassium tellurite (CT-SMAC). Non-sorbitol fermenting (NSF) Escherichia coli isolates were subjected to serotyping using commercial O157 antisera and rf b O157 gene PCR. Isolates were additionally tested for major virulence factors of EHEC including stx1 , stx2 , eae and ehly by multiplex-PCR. Results: Eighteen NSF isolates were recovered from CT-SMAC confirmed as E. coli in biochemical tests. None of the obtained isolates belonged to O157 serogroup. Overall, two isolates harbored the tested virulence genes; one isolate possessed stx2 and ehly, and the other one carried stx2 , eae and ehly . Conclusion: The results of this study indicated that cattle in Golestan province could be the reservoir for non-O157 EHEC.
کلیدواژه‌های انگلیسی مقاله Cattle,Golestan province,O157, H7,PCR
نویسندگان مقاله علیرضا کوچک زاده | alireza koochakzadeh
department of pathobiology, faculty of veterinary medicine, garmsar branch, islamic azad university, garmsar-iran and department of microbiology, faculty of veterinary medicine, university of tehran, tehran-iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

مهدی عسکری badouei | mahdi askari badouei
department of pathobiology, faculty of veterinary medicine, garmsar branch, islamic azad university, garmsar-iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

عماد مازندرانی | emad mazandarani
department of pathobiology, faculty of veterinary medicine, garmsar branch, islamic azad university, garmsar-iran.

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

امید مددگار | omid madadgar
department of microbiology, faculty of veterinary medicine, university of tehran, tehran-iran.

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/389
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