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Iranian Journal of Allergy, Asthma and Immunology، جلد ۶، شماره ۳، صفحات ۱۰۷-۱۱۴
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عنوان انگلیسی Interaction of CpG-Oligodeoxynucleotides with Toll Like Receptor 9 Induces Apoptosis and Modulates Metaloproteinase-2 Activity in Human Intestinal Epithelium
چکیده انگلیسی مقاله Recent reports have indicated different effects of immunostimulatory sequences containing CpG-Oligodeoxynucleotides (ODN) on various immune cells. However, the exact role of CpG-ODN in the human gut is unclear. In the present study, we assessed potential effects of CpG-ODN on non lymphoid cell (intestinal epithelial cell line HT-29) on a dose-response and time-course basis. Intestinal epithelial cell line HT-29 was treated with CpG-ODN (CpG 2006) and lipopolysaccharide (LPS) at 5, 10, 25, 50 μg/ ml and 1, 5, 10 μg/ ml concentrations, respectively. Following treatments, dose- response and time-course cytotoxicity using a colorimetric method, Metaloproteinase-2 (MMP-2) activity (using gelatin zymography) and apoptosis (using annexin-v flowcytometry method) assays were performed. Chloroquine treatment was also used for its inhibitory effect on endosomal acidification process to verify specific CpG-ODN and Toll Like Receptor 9 (TLR9) interactions. Cytotoxicity analysis of CpG-ODN showed that CpG-ODN increased significantly the proliferation of CpG-ODN treated cells, as compared to untreated cells, at concentrations of 10-25 μg/ml (p Collectively, our data indicated that intestinal epithelial cell line HT-29 is highly responsive to CpG effect in vitro and exhibits modified activities. The direct CpG-ODN and TLR-9 interactions in HT-29 cells could provide new approaches in malignant tumor therapeutic strategies.
کلیدواژه‌های انگلیسی مقاله CpG-ODN, HT-29, TLR9
نویسندگان مقاله محمد رضا خرمی زاده | mohammad reza khorramizadeh


شهناز حسین زاده | shahnaz hosseinzadeh


فرناز صفوی فر | farnaz safavifar


فرشید سعادت | farshid saadat


نسترن عالی زاده | nastaran aalizadeh


رضا فلک | reza falak


زهره جدلی | zohre jadali


محمد پزشکی | mohammad pezeshki


نشانی اینترنتی http://ijaai.tums.ac.ir/index.php/ijaai/article/view/173
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