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Iranian Journal of Allergy, Asthma and Immunology، جلد ۱۰، شماره ۱، صفحات ۵۳-۵۹
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عنوان انگلیسی Schwann Cell Apoptosis and p75NTR siRNA
چکیده انگلیسی مقاله The p75 pan-neurotrophin receptor (p75NTR) plays a pivotal role in linking the immune system with the nervous system. p75NTR is required for the development of several characteristic features of allergic asthma. Also p75NTR upregulated by reactive Schwann cells after peripheral nerve injury. Moreover p75NTR and RhoA play a critical role in the regulation of apoptosis. To determine whether the designed siRNA for p75NTR can downregulates both p75NTR and Rho-A at RNA level in rats and, if so, at what magnitude, Schwann cell apoptosis occurs. Isolation and purification of neonate Schwann cells were prepared from rat sciatic nerve. Specific siRNA duplex was designed for p75NTR. To investigate the role of siRNA-mediated knockdown of p75NTR, the gene expression in p75NTR was examined with reverse transcription-polymerase chain reaction (RT-PCR) and Real-Time RT-PCR. Schwann cell apoptosis was performed by Annexin and TUNEL assays after 24 hours. Following p75NTR Transfection siRNA, p75NTR gene, compared with control, was downregulated by 73%. Without using siRNA for Rho-A, Rho-A gene was downregulated by 89% at the same time. Based on Annexin assay, apoptosis of Schwann cells occurred in siRNA+NGF and control+NGF by 16.76%±2.27 and 92.39%±1.82, respectively. TUNEL data showed that apoptosis of Schwann cells occurred in siRNA and control by 12.91%±6.39 and 78.55%±11.85, respectively. Thus, p75-siRNA downregulated both p75NTR and Rho-A at RNA level in rats and showed a role on decreased cell apoptosis compared to the controls.
کلیدواژه‌های انگلیسی مقاله Apoptosis,Rho A,Schwann Cell Culture,siRNA,P75NTR
نویسندگان مقاله معصومه فیروزی | masoumeh firouzi
department of biochemistry, national institute of genetic engineering and biotechnology, tehran, iran and tissue repair lab, institute of biochemistry and biophysics, university of tehran and research centre for neural repair, university of tehran

سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

فرزانه صابونی | farzaneh sabouni
department of biochemistry, national institute of genetic engineering and biotechnology, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

عبدالخالق دیزجی | abdolkhaleg deezagi
department of biochemistry, national institute of genetic engineering and biotechnology, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

زهرا حسن نژاد پیربستی | zahra hassannejad pirbasti
tissue repair lab, institute of biochemistry and biophysics, university of tehran and research centre for neural repair, university of tehran

سازمان اصلی تایید شده: مرکز تحقیقات بیوشیمی و بیوفیزیک (Institute of biochemistry and biophysics)

فاطمه پوررجب | fatemeh poorrajab
tissue repair lab, institute of biochemistry and biophysics, university of tehran

سازمان اصلی تایید شده: مرکز تحقیقات بیوشیمی و بیوفیزیک (Institute of biochemistry and biophysics)

وفا رحیمی موقر | vafa rahimi movaghar
sina trauma and surgery research center, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijaai.tums.ac.ir/index.php/ijaai/article/view/293
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