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Iranian Journal of Allergy, Asthma and Immunology، جلد ۱۲، شماره ۴، صفحات ۳۱۲-۳۲۰
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عنوان انگلیسی Purification and identification of 72 kDa and 15 kDa allergens from Broussonetia papyrifera pollen.
چکیده انگلیسی مقاله “Broussonetia papyrifera” (Chinese mulberry) pollen is an important source of allergens in regions surrounding Shanghai, China. To identify and purify major allergens from “B. papyrifera” pollen that reacted with serum antibodies from sensitized patients, “B. papyrifera” pollen was defatted, dried, and extracted proteins were separated using SP cationic exchange or Q anionic exchange columns. Serum samples from 29 allergic patients and 4 healthy controls were collected. Allergens in eluted fractions were identified by Western blot and enzyme-linked immunosorbent assays (ELISA) using serum samples. An inhibitory assay was used to verify allergen-specific antiserum specificity. Serum IgE of 2 patients reacted with a 15 kDa protein band. The protein was eluted with 0.1M NaCl from a SP cationic exchange column. Serum samples from the same patients positively reacted with ELISA plate coated with partially purified 15 kDa protein. Serum IgE of 11 patients reacted with a 72 kDa protein band. The protein was eluted with 0.3M NaCl from a Q anionic exchange column. Serum samples from five patients positively reacted with ELISA coated with partially purified 72 kDa protein. Our preliminary purifications identified two proteins of 72 kDa and 15 kDa as allergens derived from “B. papyrifera” pollen, which reacted with allergic patients’ serum IgE.
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نویسندگان مقاله zhen chen | zhen chen
shanghai huashan hospital, fudan university, no. 12, urumqi road m , shanghai 200040 china


ning zhu | ning zhu
shanghai huashan hospital, fudan university, no. 12, urumqi road m , shanghai 200040 china


xiaodong chen | xiaodong chen
shanghai huashan hospital, fudan university, no. 12, urumqi road m , shanghai 200040 china


yi یانگ | yi yang
shanghai huashan hospital, fudan university, no. 12, urumqi road m , shanghai 200040 china


yiping li | yiping li
institute of biochemistry and cell biology, shanghai institute of life sciences, chinese academy of sciences, no. 320, yueyang road, shanghai 200031 china


zhili wu | zhili wu
institute of biochemistry and cell biology, shanghai institute of life sciences, chinese academy of sciences, no. 320, yueyang road, shanghai 200031 china


shaoxin chen | shaoxin chen
shanghai institute of pharmaceutical industry, no. 1320, peking w. road. shanghai 200040 china
نشانی اینترنتی http://ijaai.tums.ac.ir/index.php/ijaai/article/view/496
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