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Iranian Journal of Allergy, Asthma and Immunology، جلد ۱۴، شماره ۱، صفحات ۱۰۵-۱۱۲
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عنوان انگلیسی Development of a new immunochromatographic assay using gold nanoparticles for screening of IgA deficiency.
چکیده انگلیسی مقاله A new competitive immunochromatography (ICG) strip test based on polyclonal antibody (pAb) conjugated with gold nanoparticles (NPs) was developed and its applications for primary screening of immunoglobulin (Ig) A in serum were evaluated. Nanocolloidal gold as the detection reagent, with an average particle diameter of 20 nm, was synthesized and labelled pAb. The antibody-nanocolloidal gold probe was applied on the conjugate pad, and human IgA was immobilized on a nitrocellulose membrane as the capture reagent to prepare the ICG strip test. It took only 10 minutes to accomplish a semi-quantitative detection of serum IgA in this assay. In the optimized investigational conditions, the ICG strip test could distinguish human serum IgA in the range from 1 to 270 ng/mL with a detection limit of 5 ng/mL. The reliability of testing procedures was examined by performing the ICG strip test with 11 serum samples and comparing the results with those obtained via enzyme-linked immunosorbent assay (ELISA). The ICG strip was sufficiently sensitive and accurate for a rapid screening of IgA in human serum.
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نویسندگان مقاله سعید گودرزی | saeid goudarzi
clinical nanomedicine laboratory, ent- head amp;amp; neck research center, hazrate rasoul akram hospital, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

آنیتا احمدی | anita ahmadi
biosensor research center, endocrinology and metabolism molecular-cellular sciences institute, tehran university of medical sciences, tehran, iran and endocrinology and metabolism research center, endocrinology and metabolism research institute, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد فرهادی | mohammad farhadi
clinical nanomedicine laboratory, ent- head amp;amp; neck research center, hazrate rasoul akram hospital, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

سید کامران کامروا | seyed kamran kamrava
clinical nanomedicine laboratory, ent- head amp;amp; neck research center, hazrate rasoul akram hospital, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

شیوا ثقفی | shiva saghafi
immunology, asthma amp;amp; allergy research institute, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

کبری امیدفر | kobra omidfar
biosensor research center, endocrinology and metabolism molecular-cellular sciences institute, tehran university of medical sciences, tehran, iran and endocrinology and metabolism research center, endocrinology and metabolism research institute, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijaai.tums.ac.ir/index.php/ijaai/article/view/405
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