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Iranian Journal of Allergy, Asthma and Immunology، جلد ۲۲، شماره ۳، صفحات ۲۳۳-۲۴۴
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عنوان انگلیسی Exosomes from Adipose Tissue-derived Mesenchymal Stem Cells Induce Regulatory T Cells in COVID‐19 Patients
چکیده انگلیسی مقاله An imbalance between regulatory T (Treg) and T-helper (Th)-17 cells has been implicated in the pathogenesis of coronavirus disease 2019 (COVID-19). Mesenchymal stem cells (MSCs) exert immunomodulatory properties through secreting exosomes. This study aimed to assess the effect of MSC-derived exosomes (MSC-Exo) on the differentiation of peripheral blood mononuclear cells (PBMCs) into  Tregs from patients with COVID-19. Exosomes were isolated from adipose tissue–derived MSCs. PBMCs were separated from the whole blood of COVID-19 patients (n=20). Treg frequency was assessed before and 48 hours after treatment of PBMCs with MSC-Exo using flow cytometry. Expression of FOXP3 and cytokine genes, and the concentration of cytokines associated with Tregs, were assessed before and after treatment with MSC-Exo. The frequency of CD4+CD25+CD127-  Tregs was significantly higher after treating PBMCs with MSC-Exo (6.695±2.528) compared to before treatment (4.981±2.068). The expressions of transforming growth factor (TGF)-β1, interleukin (IL)-10, and FOXP3 were significantly upregulated in MSC-Exo–treated PBMCs. The concentration of IL‐10 increased significantly after treatment (994.7±543.9 pg/mL) of PBMCs with MSC-Exo compared with before treatment (563.5±408.6 pg/mL). The concentration of TGF-β was significantly higher in the supernatant of PBMCs after treatment with MSC-Exo (477.0±391.1 pg/mL) than PBMCs before treatment (257.7±226.3 pg/mL). MSC-Exo has the potential to raise anti-inflammatory responses by induction of  Tregs, potentiating its therapeutic effects in COVID-19.
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نویسندگان مقاله | Morteza Motallebnezhad
Health Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran


| Ali Hazrati
Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Hadi Esmaeili Gouvarchin Ghaleh
Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran


| Nematollah Jonaidi-Jafari
Health Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran


| Sanaz Abbaspour-Aghdam
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Kosar Malekpour
Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Mehdi Yousefi
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Hossein Samadi Kafil
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Farhad Jadidi-Niaragh
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Leila Roshangar
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


| Hamed Valizadeh
Tuberculosis and Lung Disease Research Center of Tabriz University of Medical Sciences, Tabriz, Iran


| Morteza Izadi
Health Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran


| Majid Ahmadi
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
نشانی اینترنتی https://ijaai.tums.ac.ir/index.php/ijaai/article/view/3666
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