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Iranian Journal of Allergy, Asthma and Immunology، جلد ۱۹، شماره ۱، صفحات ۸۴-۹۳
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عنوان انگلیسی In Vitro Effects of Curcumin on Transforming Growth Factor-β-mediated Non-Smad Signaling Pathway, Oxidative Stress, and Pro‐inflammatory Cytokines Production with Human Vascular Smooth Muscle Cells
چکیده انگلیسی مقاله Transforming growth factor-β (TGF-β) induces pro-inflammatory cytokines expression including interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) and these cytokines are associated with the development of atherosclerosis. Curcumin has anti-atherogenic effects and anti-inflammatory properties in the vascular wall, but the relative mechanisms are almost unknown. In the present study, we investigate the effect of curcumin on modulating the pro-inflammatory action of TGF-β in human vascular smooth muscle cells (VSMCs) and its molecular mechanisms. Cultured VSMCs were seeded into several groups: a control group (untreated group), a group treated with TGF-β, and several groups treated with TGF-β plus inhibitors. The cells were pre-treated with diphenyleneiodonium chloride, DPI, (20 μM), curcumin (5, 10 and 20 μM) and N-Acetyl-L-Cysteine, NAC, (10 mM) and then TGF-β (5 ng/mL) was added to the culture medium. The mRNA levels of IL-6 and TNF-α were detected by quantitative Real-Time Polymerase Chain Reaction. For monitoring the Smad2 linker region phosphorylation (pSmad2L), the western-blotting technique was applied and reactive oxygen species (ROS) generation was measured by utilizing 2′,7′-dichlorofluorescein diacetate-based assay. TGF-β increased the mRNA expression of IL-6 (p=0.02 and p=0.001) and TNF-α (p =0.014 and p = 0.001) in a time-dependent manner, ROS production (p=0.03) and Smad2L phosphorylation (p=0.015). Pre-treatment with curcumin, DPI and NAC inhibited TGF-β–induced IL-6 (p=0.04) and TNF-α (p=0.001) mRNA expression, Smad2L phosphorylation (p=0.02) and ROS production (0.03). Pharmacological inhibition by Curcumin blocks TGF-β–induced ROS production, Smad2L phosphorylation, and IL-6 and TNF-α mRNA expression in human VSMCs.
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نویسندگان مقاله | Amirhooman Asadi
Veterinary Medicine, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran


| Davood Yaghobi Nezhad
Department of Agriculture Biotechnology, Faculty of Agriculture, Payame Noor University, Tehran, Iran


| Amirreza Rafie Javazm
Student Research Committee, Faculty of Nursing and Midwifery, Shahid Sadoughi University of Medical Sciences, Yazd, Iran


| Parisa Khanicheragh
Department of Clinical Biochemistry, Lorestan University of Medical Sciences, Khorram abad, Iran


| Ladan Mashouri
Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran


| Fatemeh Shakeri
Jahrom University of Medical Sciences, Jahrom, Iran


| Mojtaba Abbasi
Veterinary Medicine, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran


| Mahya Sadat Afrazian
Department of Biology, Faculty of Basic Sciences, University of Shiraz, Shiraz, Iran


| Zahra Niknam
Student Research Committee, Department of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapor University of Medical Science, Ahvaz, Iran


| Omid Abazari
Department of Biochemistry, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
نشانی اینترنتی http://ijaai.tums.ac.ir/index.php/ijaai/article/view/2472
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