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Iranian Journal of Allergy, Asthma and Immunology، جلد ۱۸، شماره ۳، صفحات ۲۸۹-۲۹۹
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عنوان انگلیسی Isolation of a Novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library
چکیده انگلیسی مقاله Vascular endothelial growth factor receptor 2 (VEGFR-2) is known as one of the important antigens playing a vital role in angiogenesis. In this study, phage display technology (PDT) was used to produce a single-chain variable fragment (scFv) antibody against a region of the domain 3 in VEGFR-2 called kinase insert domain receptor 3 (KDR3). After designing the KDR3 peptide and biopanning, a colony was chosen for scFv antibody expression. Following expression and purification; western blotting, dot blotting and immunofluorescence (IF) were used to evaluate the antibody function. Surface plasmon resonance (SPR) was also employed to measure affinity of produced antibody. Once a colony was selected and transferred to the expression host, the scFv antibody was expressed in the expected range of 28 kDa. Using a designed chromatography column, antibody purification was found to be about 95%. In this study, a novel scFv with the capability of binding to KDR3 was isolated and purified and its intracellular function was investigated and verified.
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نویسندگان مقاله | Shirafkan Kordi
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2 Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran


| Mohammad Rahmati-Yamchi
Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran


| Mehdi Asghari Vostakolaei
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran


| Ali Etemadi
Department of Medical Biotechnology, Tehran University of Medical Sciences, Tehran, Iran


| Abolfazl Barzegari
Research Centre for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran


| Jalal Abdolalizadeh
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Paramedical Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
نشانی اینترنتی http://ijaai.tums.ac.ir/index.php/ijaai/article/view/2121
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